The present manuscript describes simple, sensitive, rapid, accurate, precise and economical derivative spectroscopic method for the simultaneous determination of Eperisone hydrochloride (EPE) and Diclofenac sodium (DIC) in synthetic mixture. Derivative spectroscopy offers a useful approach for the analysis of drugs in mixtures. In this study a first-derivative spectroscopic method was used for simultaneous determination of Eperisone hydrochloride and diclofenac sodium using the zero-crossing technique. The measurements were carried out at wavelengths of 281 nm and 225 nm for Eperisone hydrochloride and Diclofenac sodium respectively. The method was found to be linear (r2>0.996) in the range of 2- 16 μg/ml for Eperisone hydrochloride at 281 nm. The linear correlation was obtained (r2>0.999) in the range of 2-16 μg/ml for Diclofenac sodium at 225 nm. The limit of determination was 0.3930 and 0.4095 μg/ml for Eperisone hydrochloride and Diclofenac sodium respectively. The limit of quantification was 1.1911 and 1.2409 μg/ml. The method was successfully applied for simultaneous determination of Eperisone hydrochloride and Diclofenac sodium in binary mixture.
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